diff --git a/.changeset/two-needles-buy.md b/.changeset/two-needles-buy.md
new file mode 100644
index 000000000..ebf000cfc
--- /dev/null
+++ b/.changeset/two-needles-buy.md
@@ -0,0 +1,6 @@
+---
+'astro': minor
+'@astrojs/node': minor
+---
+
+Add support for serving well-known URIs with the @astrojs/node SSR adapter
diff --git a/packages/astro/src/core/build/static-build.ts b/packages/astro/src/core/build/static-build.ts
index 13f40c8da..747ef360f 100644
--- a/packages/astro/src/core/build/static-build.ts
+++ b/packages/astro/src/core/build/static-build.ts
@@ -191,7 +191,7 @@ async function clientBuild(
 	if (!input.size) {
 		// If SSR, copy public over
 		if (ssr) {
-			await copyFiles(settings.config.publicDir, out);
+			await copyFiles(settings.config.publicDir, out, true);
 		}
 
 		return null;
@@ -318,9 +318,10 @@ async function cleanServerOutput(opts: StaticBuildOptions) {
 	}
 }
 
-async function copyFiles(fromFolder: URL, toFolder: URL) {
+async function copyFiles(fromFolder: URL, toFolder: URL, includeDotfiles = false) {
 	const files = await glob('**/*', {
 		cwd: fileURLToPath(fromFolder),
+		dot: includeDotfiles,
 	});
 
 	await Promise.all(
diff --git a/packages/astro/test/fixtures/static-build-ssr/public/.well-known/apple-app-site-association b/packages/astro/test/fixtures/static-build-ssr/public/.well-known/apple-app-site-association
new file mode 100644
index 000000000..daae260f1
--- /dev/null
+++ b/packages/astro/test/fixtures/static-build-ssr/public/.well-known/apple-app-site-association
@@ -0,0 +1,3 @@
+{
+  "applinks": {}
+}
diff --git a/packages/astro/test/static-build.test.js b/packages/astro/test/static-build.test.js
index 7e1c4c872..db35ef991 100644
--- a/packages/astro/test/static-build.test.js
+++ b/packages/astro/test/static-build.test.js
@@ -191,6 +191,8 @@ describe('Static build SSR', () => {
 			root: './fixtures/static-build-ssr/',
 		});
 		await fixture.build();
-		const asset = await fixture.readFile('/client/nested/asset2.txt');
+
+		await fixture.readFile('/client/nested/asset2.txt');
+		await fixture.readFile('/client/.well-known/apple-app-site-association');
 	});
 });
diff --git a/packages/integrations/node/src/http-server.ts b/packages/integrations/node/src/http-server.ts
index bdd628c29..19e33c84a 100644
--- a/packages/integrations/node/src/http-server.ts
+++ b/packages/integrations/node/src/http-server.ts
@@ -17,10 +17,11 @@ export function createServer(
 ) {
 	const listener: http.RequestListener = (req, res) => {
 		if (req.url) {
-			const pathname = '/' + removeBase(req.url);
+			let pathname = removeBase(req.url);
+			pathname = pathname[0] === '/' ? pathname : '/' + pathname;
 			const stream = send(req, encodeURI(pathname), {
 				root: fileURLToPath(client),
-				dotfiles: 'deny',
+				dotfiles: pathname.startsWith('/.well-known/') ? 'allow' : 'deny',
 			});
 
 			let forwardError = false;
diff --git a/packages/integrations/node/test/fixtures/well-known-locations/package.json b/packages/integrations/node/test/fixtures/well-known-locations/package.json
new file mode 100644
index 000000000..f018b6ec7
--- /dev/null
+++ b/packages/integrations/node/test/fixtures/well-known-locations/package.json
@@ -0,0 +1,9 @@
+{
+  "name": "@test/well-known-locations",
+  "version": "0.0.0",
+  "private": true,
+  "dependencies": {
+    "astro": "workspace:*",
+    "@astrojs/node": "workspace:*"
+  }
+}
diff --git a/packages/integrations/node/test/fixtures/well-known-locations/public/.hidden/file.json b/packages/integrations/node/test/fixtures/well-known-locations/public/.hidden/file.json
new file mode 100644
index 000000000..0967ef424
--- /dev/null
+++ b/packages/integrations/node/test/fixtures/well-known-locations/public/.hidden/file.json
@@ -0,0 +1 @@
+{}
diff --git a/packages/integrations/node/test/fixtures/well-known-locations/public/.well-known/apple-app-site-association b/packages/integrations/node/test/fixtures/well-known-locations/public/.well-known/apple-app-site-association
new file mode 100644
index 000000000..daae260f1
--- /dev/null
+++ b/packages/integrations/node/test/fixtures/well-known-locations/public/.well-known/apple-app-site-association
@@ -0,0 +1,3 @@
+{
+  "applinks": {}
+}
diff --git a/packages/integrations/node/test/well-known-locations.test.js b/packages/integrations/node/test/well-known-locations.test.js
new file mode 100644
index 000000000..31f31bacd
--- /dev/null
+++ b/packages/integrations/node/test/well-known-locations.test.js
@@ -0,0 +1,45 @@
+import nodejs from '../dist/index.js';
+import { loadFixture } from './test-utils.js';
+import { expect } from 'chai';
+
+describe('test URIs beginning with a dot', () => {
+	/** @type {import('./test-utils').Fixture} */
+	let fixture;
+
+	before(async () => {
+		fixture = await loadFixture({
+			root: './fixtures/well-known-locations/',
+			output: 'server',
+			adapter: nodejs({ mode: 'standalone' }),
+		});
+		await fixture.build();
+	});
+
+	describe('can load well-known URIs', async () => {
+		let devPreview;
+
+		before(async () => {
+			devPreview = await fixture.preview();
+		});
+
+		after(async () => {
+			await devPreview.stop();
+		});
+
+		it('can load a valid well-known URI', async () => {
+			const res = await fixture.fetch('/.well-known/apple-app-site-association');
+
+			expect(res.status).to.equal(200);
+
+			const json = await res.json();
+
+			expect(json).to.deep.equal({ applinks: {} });
+		});
+
+		it('cannot load a dot folder that is not a well-known URI', async () => {
+			const res = await fixture.fetch('/.hidden/file.json');
+
+			expect(res.status).to.equal(404);
+		});
+	});
+});
diff --git a/pnpm-lock.yaml b/pnpm-lock.yaml
index e8d3b7ec9..c10f8c04b 100644
--- a/pnpm-lock.yaml
+++ b/pnpm-lock.yaml
@@ -3097,6 +3097,14 @@ importers:
       '@astrojs/node': link:../../..
       astro: link:../../../../../astro
 
+  packages/integrations/node/test/fixtures/well-known-locations:
+    specifiers:
+      '@astrojs/node': workspace:*
+      astro: workspace:*
+    dependencies:
+      '@astrojs/node': link:../../..
+      astro: link:../../../../../astro
+
   packages/integrations/partytown:
     specifiers:
       '@builder.io/partytown': ^0.7.4